Mechanism of Huangqin Qingre Chubi Capsules in improving rheumatoid arthritis based on METTL3-SFRP4/Wnt/β-catenin pathway

• Published in: Zhongguo zhongyao zazhi Vol. 49; no. 11; p. 3081
• Main Authors: Wang, Yu-Ting, Wu, Ya-Jie, Huang, Yu-Rong, Xue, Qiu-Yun, Cheng, Cheng-Long, Miao, Cheng-Gui, Chen, Guang-Liang
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2024
• Subjects: Animals; Arthritis, Rheumatoid - drug therapy; Arthritis, Rheumatoid - genetics; Arthritis, Rheumatoid - metabolism; beta Catenin - genetics; beta Catenin - metabolism; Capsules; Drugs, Chinese Herbal - administration & dosage; Drugs, Chinese Herbal - pharmacology; Humans; Male; Methyltransferases - genetics; Methyltransferases - metabolism; Proto-Oncogene Proteins; Rats; Rats, Sprague-Dawley; Synoviocytes - drug effects; Synoviocytes - metabolism; Wnt Signaling Pathway - drug effects; SFRP4; Huangqin Qingre Chubi Capsules; rheumatoid arthritis; Wnt/β-catenin; METTL3
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20240229.501

The effect and mechanism of Huangqin Qingre Chubi Capsules(HQC) on rheumatoid arthritis(RA) were studied.Seventy male SPF rats were randomly divided into normal group, model group, low-(0. 18 g·kg~(-1)), middle-(0. 36 g·kg~(-1)), and high-(0. 72 g·kg~(-1)) dose groups of HQC, methotrexate group(MTX, 0. 75 mg·kg~(-1)), and negative control group(NC group, model +saline). Adjuvant arthritis fibroblast-like synoviocytes(AA-FLS) were divided into normal group, model group, low-, middle-, and high-dose groups of HQC, and negative control group. RT-qPCR and Western blot were used to detect the m RNA and protein expressions of METTL3, SFRP4, β-catenin, CCND1, c-Myc, MMP3, and fibronectin. The protein expression of MMP3 and β-catenin was detected by immunofluorescence. The gene expression level of METTL3 on AA-FLS was knocked down to further examine the expression of each gene. ELISA measured the levels of IL-1β, IL-6, and IL-8. The results showed that compared with the normal group, rats in the model group found red