Study on mechanism of enhancing antioxidant activity in fermented Ziziphi Spinosae Semen with Poria cocos

• Published in: Zhongguo zhongyao zazhi Vol. 46; no. 3; p. 620
• Main Authors: Li, Ze, Xie, Yu-Jun, Duan, Hui-Zhu, Cui, Xiao-Fang, Pei, Xiang-Ping, Yan, Yan, DU, Chen-Hui
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2021
• Subjects: Antioxidants; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Poria; Semen; Wolfiporia; Ziziphus; 1H-NMR metabonomics; fermentation; antioxidant; Ziziphi Spinosae Semen; Poria cocos
• DOI: 10.19540/j.cnki.cjcmm.20201012.202
• ISSN: 1001-5302

In this study, the antioxidant property changes in fermented Ziziphi Spinosae Semen(FZSS) with Poria cocos were analyzed by DPPH, ABTS and FRAP methods. Then the content determination of active ingredients and ~1H nuclear magnetic resonance(~1H-NMR) spectroscopy were also used to investigate the mechanism of FZSS with P. cocos in enhancing the antioxidant activity. The results showed that the content of active ingredients such as total phenols, total saponins and total polysaccharides were significantly increased during the fermentation time. The results of ~1H-NMR metabonomics showed that the contents of amino acids such as leucine, lysine, valine and alanine, nitrogen compounds such as creatine, creatinine, and betaine, and secondary metabolites, for instance, jujuboside A and spinosin were higher after fermentation, and above components showed positive correlation with antioxidant capacity in Pearson correlation analysis. Therefore, it was inferred that the enhancement of antioxidant activity of FZSS may b