Chromogenic in situ hybridization in diagnostics of Epstein-Barr-virus infection in chronic tonsillitis

In the issue we demonstrate results of Epstein-Barr virus (EBV) detection by chromogenic in situ hybridization (CISH) in epithelial and lymphoid cells of the palatine tonsil in patients with chronic tonsillitis. Virus genome detections were performed using RNA-probes with digoxigenin-labeled oligonu...

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Bibliographic Details
Published inArkhiv patologii Vol. 74; no. 2; p. 19
Main Authors Bykova, V P, Khafizova, F A, Kalinina, D V, Daĭkhes, N A
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.2012
Subjects
Chronic Disease
DNA, Viral - genetics
DNA, Viral - metabolism
Epstein-Barr Virus Infections - diagnosis
Epstein-Barr Virus Infections - genetics
Epstein-Barr Virus Infections - metabolism
Epstein-Barr Virus Infections - pathology
Female
Herpesvirus 4, Human - genetics
Herpesvirus 4, Human - metabolism
Humans
In Situ Hybridization - methods
Male
RNA, Viral - genetics
RNA, Viral - metabolism
Tonsillitis - diagnosis
Tonsillitis - genetics
Tonsillitis - metabolism
Tonsillitis - pathology
Tonsillitis - virology
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Summary:In the issue we demonstrate results of Epstein-Barr virus (EBV) detection by chromogenic in situ hybridization (CISH) in epithelial and lymphoid cells of the palatine tonsil in patients with chronic tonsillitis. Virus genome detections were performed using RNA-probes with digoxigenin-labeled oligonucleotides which target EBV RNA, notably RNA-transcripts of virus genomic DNA. The obtained data confirm the virus lymphotropism and also tropism to epithelial cells of both surface and cryptal epithelium of the palatine tonsil. CISH method in combination with immunohistochemical identification of virus protein products opens new possibilities for clinicopathological monitoring of the different clinical forms of the chronic tonsillitis, as well as new horizon for understanding intrinsic role of EBV in tonsillar pathology.
ISSN:0004-1955