Cloning and quantitative expression analysis of GMPP gene from Dendrobium huoshanense

In order to understand the function of GDP-mannose pyrophosphorylase(GMPP) function and its regulation in polysaccharide biosynthesis mechanism in Dendrobium. D. huoshanense was used to clone GMPP gene. GMPP gene expression in D. huoshanense,D. officinale and D. moniliforme was also determined by qP...

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Published inZhongguo zhongyao zazhi Vol. 44; no. 8; p. 1552
Main Authors Han, Rong-Chun, Liu, Lu-Lu, Liu, Jun-Lin, Xie, Dong-Mei, Peng, Dai-Yin, Yu, Nian-Jun
Format Journal Article
LanguageChinese
Published China 01.04.2019
Subjects
qPCR
Dendrobium huoshanense
gene expression
GMPP
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Summary:In order to understand the function of GDP-mannose pyrophosphorylase(GMPP) function and its regulation in polysaccharide biosynthesis mechanism in Dendrobium. D. huoshanense was used to clone GMPP gene. GMPP gene expression in D. huoshanense,D. officinale and D. moniliforme was also determined by qPCR. The results showed that the length of D. huoshanense GMPP gene c DNA sequence is 1 867 bp,containing 1 245 bp open reading frame(ORF),encoding 415 amino acids. Phylogenetic tree analysis showed that D. huoshanense,D. officinale and D. moniliforme are closely related with GMPP taken into consideration. Bioinformatics analysis demonstrated that GMPP sequence similarity among the three species reached as high as 99%. qPCR results indicated that GMPP genes was highly expressed in stem of D. huoshanense compared with its leaf,flower and root. According to GMPP gene expression profile in D. huoshanense,D. officinale and D. moniliforme grown in Huoshan area,it was clear that GMPP in D. huoshanense showed the highest e
ISSN:1001-5302
DOI:10.19540/j.cnki.cjcmm.20190129.013