Estimation of atherogenic immune complexes containing modified lipoproteins in complement fixation tests

A method for determining atherogenicity of the immune complexes containing multiple modified low-density lipoprotein (mmLDL) in complement fixation test has been found. In the proposed method, the precipitate immune complexes containing mmLDL (IC mmLDL) was prepared from human serum by treating it w...

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Published inPatologicheskaia fiziologiia i eksperimental'naia terapiia no. 4; p. 133
Main Authors Shoibonov, B B, Kravchenko, M A, Baronets, V Y, Tolpygo, S M, Kostyreva, M V, Shabalina, A A, Zamolodchikova, T S, Kotov, A V, Panchenko, L F
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.10.2014
Subjects
Animals
Antigen-Antibody Complex - blood
Atherosclerosis - blood
Atherosclerosis - diagnostic imaging
Atherosclerosis - immunology
Carotid Artery, Common - diagnostic imaging
Carotid Intima-Media Thickness
Complement Fixation Tests
Guinea Pigs
Humans
Lipoproteins, LDL - blood
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Summary:A method for determining atherogenicity of the immune complexes containing multiple modified low-density lipoprotein (mmLDL) in complement fixation test has been found. In the proposed method, the precipitate immune complexes containing mmLDL (IC mmLDL) was prepared from human serum by treating it with buffer (8.3% of th PEG 3350 and 3.3% PVP 12600 th in the ratio 1: 1.2) for 10 min at 23 °C. IC mmLDL aggregates were separated by centrifugation at 3100g for 10 min at 23 °C. The precipitate IC mmLDL was dissolved in buffer without PEG and PVP, cholesterol content and the degree of binding of guinea pig complement were measured. Atherogenicity of the IC mmLDL was registered as the ratio of the degree of complement binding to cholesterol in the the immune complexes.
ISSN:0031-2991