Alcohol extract of root and root bark of Toddalia asiatica alleviates CIA in rats through anti-inflammatory and proapoptotic effects

• Published in: Zhongguo zhongyao zazhi Vol. 48; no. 8; p. 2203
• Main Authors: Zhang, Zong-Xing, Jiang, Lu, Liu, Dao-Zhong, Tao, Bo-Nan, Hou, Zi-Ming, Tian, Meng-Jie, Feng, Jia, Yuan, Lin
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2023
• Subjects: apoptosis; CIA; PI3K/Akt signaling pathway; rheumatoid arthritis; alcohol extract of root and root bark of Toddalia asiatica
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20230103.706

This study aims to investigate the therapeutic effect of alcohol extract of root and root bark of Toddalia asiatica(TAAE) on collagen-induced arthritis(CIA) in rats through phosphatidylinoinosidine-3 kinase/protein kinase B(PI3K/Akt) signaling pathway. To be specific, CIA was induced in rats, and then the rats were treated(oral, daily) with TAAE and Tripterygium Glycoside Tablets(TGT), respectively. The swelling degree of the hind leg joints was scored weekly. After 35 days of administration, the histopathological changes were observed based on hematoxylin and eosin(HE) staining. Enzyme-linked immunosorbent assay(ELISA) was employed to detect the levels of cytokines [tumor necrosis factor-α(TNF-α), interleukin(IL)-6)]. Terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL) staining was performed to detect the apoptosis of synoviocytes in rats. Western blot was used to detect the expression levels of apoptosis-related proteins B-cell lymphoma 2(Bcl-2)-associated X(Bax), Bcl-2, and caspase-3 and pa