The Role and Mechanism of MiR-451 in Multidrug Resistance of Leukemia Cell Line K562/A02

To detect the differential expressions of miR-451, and in drug-sensitive leukemia cell line K562 and drug-resistant cell line K562/A02, and explore the regulatory relationship between miR-451 and the expressions of and , and the mechanism of miR-451 involved in drug resistance in leukemia. CCK-8 ass...

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Published inZhongguo shi yan xue ye xue za zhi Vol. 31; no. 3; p. 685
Main Authors Feng, Yan-Li, Su, Bao-Xiong, Ge, Fan-Mei, Dai, Chong-Wen
Format Journal Article
LanguageChinese
Published China 01.06.2023
Subjects
Doxorubicin - pharmacology
Drug Resistance, Multiple - genetics
Drug Resistance, Neoplasm - genetics
Humans
K562 Cells
Leukemia - genetics
MicroRNAs - genetics
RNA, Messenger
leukemia
multidrug resistance
miR-451
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Summary:To detect the differential expressions of miR-451, and in drug-sensitive leukemia cell line K562 and drug-resistant cell line K562/A02, and explore the regulatory relationship between miR-451 and the expressions of and , and the mechanism of miR-451 involved in drug resistance in leukemia. CCK-8 assay was used to detect the drug resistance of K562/A02 and K562 cells. Quantitative Real-time PCR (qRT-PCR) was used to verify the differential expressions of miR-451 in K562 and K562/A02 cells. MiR-451 mimic and negative control (miR-NC), miR-451 inhibitor and negative control (miR-inNC) were transfected into K562 and K562/A02 cells respectively, then qRT-PCR and Western blot were used to detect the expression levels of mRNA and protein of and in K562 and K562/A02 cells and the transfected groups. The drug resistance of K562/A02 cells to adriamycin was 177 times higher than that of its parent cell line K562. Compared with K562 cells, the expression of miR-451 in K562/A02 cells was significantly higher ( <0.001), an
ISSN:1009-2137
DOI:10.19746/j.cnki.issn.1009-2137.2023.03.011