Synergistic effect on biosynthesis of Panax notoginseng saponins by overexpressing a transcription factor PnbHLH and RNA interference of cycloartenol synthase gene

• Published in: Zhongguo zhongyao zazhi Vol. 46; no. 1; p. 94
• Main Authors: Jiang, Li, Yu, Yi-Lin, Jiang, Min, Cui, Xiu-Ming, Liu, Di-Qiu, Ge, Feng
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2021
• Subjects: Intramolecular Transferases; Panax notoginseng; RNA Interference; Saponins; Transcription Factors - genetics; Panax notoginseng; Panax; RNA interference; saponin; transcription factor bHLH
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20201011.101

This study cloned the transcription factor gene PnbHLH which held an open reading frame of 966 bp encoding 321 amino acids. This study constructed the overexpression vector of transcription factor PnbHLH of Panax notoginseng. The combination of PnbHLH overexpression and RNAi of the key enzyme gene PnCAS involved in the phytosterol biosynthesis was achieved in P. notoginseng cells, thus exploring the biosynthetic regulation of P. notoginseng saponins(PNS) by the synergistic effect of PnbHLH overexpression and PnCAS RNAi. The results showed that the PnbHLH transcription factor interacted with the promoters of key enzyme genes PnDS, PnSS and PnSE in the biosynthetic pathway of PNS, and then regulated the expression levels of key enzyme genes and affected the biosynthesis of saponins indirectly. Further study indicated that the synergistic effect of PnbHLH overexpression and PnCAS RNAi was a more effective approach to regulate the biosynthesis of saponins. Compared with the wild type and PnCAS RNAi cells of P. no