Preliminary study on the diagnostic value of serum-derived exosomal lncRNA in epithelial ovarian cancer

• Published in: Chung-hua fu chʿan kʿo tsa chih Vol. 57; no. 3; p. 198
• Main Authors: Li, Y W, Li, L
• Format: Journal Article
• Language: Chinese
• Published: China 25.03.2022
• Subjects: Biomarkers, Tumor - genetics; Carcinoma, Ovarian Epithelial - genetics; Carcinoma, Ovarian Epithelial - pathology; China; DNA-Binding Proteins; Female; Humans; Ovarian Neoplasms - diagnosis; Ovarian Neoplasms - genetics; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Serum - metabolism; Transcription Factors; China
• ISSN: 0529-567X
• DOI: 10.3760/cma.j.cn112141-20220114-00022

To investigate the diagnostic value of long noncoding RNA (lncRNA) extracted from serum exosomes in epithelial ovarian cancer (EOC). (1) Patients with ovarian tumors who were hospitalized in the Affiliated Tumor Hospital of Guangxi Medical University from August 2018 to December 2019, including 35 cases of EOC patients (malignant group) and 20 cases of benign ovarian tumor patients (benign group) were collected; during the same period, 15 healthy women (normal group) who underwent physical examination in the Affiliated Tumor Hospital of Guangxi Medical University were used as controls. Fasting venous blood serum was collected from the above three groups of women, and serum exosomes were isolated and purified using commercial kits. The morphology of exosomal particles was observed with transmission electron microscope, and the particle size distribution of the exosomes was detected by NanoSight technology. The expression of specific proteins cluster of differentiation (CD) , CD , and tumor susceptibility gene