Relationship between expression of NLRP3 inflammasome and improvement of macular structure in patients with wet age-related macular degeneration after anti-vascular endothelial growth factor therapy

• Published in: Zhong hua yi xue za zhi Vol. 103; no. 4; p. 265
• Main Authors: Che, J B, Wang, J M, Gao, J, Han, W H, Zhang, J B
• Format: Journal Article
• Language: Chinese
• Published: China 31.01.2023
• Subjects: Aged; Caspase 1 - metabolism; Female; Humans; Inflammasomes - metabolism; Interleukin-18; Interleukin-1beta - metabolism; Macular Degeneration - drug therapy; Macular Degeneration - metabolism; Male; Middle Aged; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; RNA, Messenger; Tumor Necrosis Factor-alpha - metabolism; Vascular Endothelial Growth Factor A - antagonists & inhibitors
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.cn112137-20220823-01792

To explore the relationship between expression of nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome and improvement of macular structure in patients with wet age-related macular degeneration (wAMD) after anti-vascular endothelial growth factor (VEGF) therapy. A before-after study was carried out. A total of 110 patients (110 eyes) with wAMD who were admitted to Department of Ophthalmology, People's Hospital Affiliated to Shandong First Medical University between August 2019 and December 2021 were enrolled, and all patients were given vitreous injection of anti-VEGF drug (ranibizumab or bevacizumab). The aqueous humor was collected to detect mRNA levels of NLRP3, cysteinyl aspartate specific protease-1 (Caspase-1), apoptosis-associated speck-like protein (ASC) and interleukin (IL) 1β by fluorescence quantitative PCR. The levels of IL-1β, IL-18, tumor necrosis factor α (TNF-α) and VEGF in aqueous humor were detected by enzyme-linked immunosorbent assay (ELISA). The correlati