Mechanisms of Wandai Decoction in Improving Vaginal Flora of Vulvovaginal Candidiasis of the Spleen Deficiency and Excessive Dampness Type: A Study Based on Metagenomics and Metabolomics

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 56; no. 1; p. 68
• Main Authors: Han, Yue, Shen, Su, Fu, Jie, Ren, Qingling
• Format: Journal Article
• Language: Chinese
• Published: China 20.01.2025
• Subjects: Adult; Candidiasis, Vulvovaginal - drug therapy; Candidiasis, Vulvovaginal - microbiology; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; Female; Fluconazole - therapeutic use; Humans; Metabolomics; Metagenomics; Random Allocation; Vagina - microbiology; Young Adult; Vulvovaginal candidiasis; Metabolomics; Metagenomics; Vaginal flora; Wandai Decoction
• ISSN: 1672-173X
• DOI: 10.12182/20250160110

To explore the mechanism by which Wandai Decoction prevents and treats vulvovaginal candidiasis (VVC) of the spleen deficiency and excessive dampness type and restores the vaginal flora structure, and to identify the potential metabolic pathways involved using metagenomics and metabolomics. Twenty VVC patients who met the inclusion criteria were randomly assigned to a Wandai Decoction group and a fluconazole group ( = 10 in each group). Subjects in the fluconazole group were given a single oral dose of 150 mg fluconazole, while those in the Wandai Decoction group took the Wandai Decoction orally for 14 days. The vulvovaginal signs and symptoms (VSS) scores of both patient groups were evaluated before and after treatment. Vaginal secretions were collected before and after treatment. The Illumina sequencing and the liquid chromatography with tandem mass spectrometry (LC-MS/MS) platform were used to conduct metagenomic and metabolomics analyses of the vaginal secretions, respectively. The VSS score results showe