Calf blood hemodializate reduces neurotoxic effect of hydrogen peroxide on human neuroblastoma cells

• Published in: Vestnik Rossiĭskoĭ akademii medits︠i︡nskih nauk no. 9-10; p. 10
• Main Authors: Yurinskaya, M M, Vinokurov, M G, Astashkin, E I, Grachev, S V, Orekhova, N S, Novikova, A N, Sokolova, I N
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 2014
• Subjects: Animals; Apoptosis - drug effects; Cattle; Cell Line, Tumor; Enzyme Inhibitors - pharmacology; Flavonoids - pharmacology; Humans; Hydrogen Peroxide - toxicity; Imidazoles - pharmacology; MAP Kinase Kinase 4 - antagonists & inhibitors; MAP Kinase Kinase 4 - metabolism; Neuroblastoma - drug therapy; Neuroblastoma - metabolism; Neuroblastoma - pathology; Neurotoxicity Syndromes - pathology; p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors; p38 Mitogen-Activated Protein Kinases - metabolism; Phosphatidylinositol 3-Kinase - metabolism; Pyridines - pharmacology; Reactive Oxygen Species - metabolism; Renal Dialysis; Signal Transduction - drug effects
• ISSN: 0869-6047

Our aim was to study the effect of calf blood gemodializat on apoptosis and intracellular signaling pathways of neuroblastoma cells SK-N-SH human. Apoptosis was recorded by fluorescent microscopy using Hoechst 33342. Necrosis cells was monitored by propidium iodide. The fluorescence of the cells was recorded on a fluorescence inverted microscope Keyence BZ8100 (Japan). Formation of reactive oxygen species (ROS) in the cells of SK-N-SH was determined using nitroblue tetrazolium by absorbance at 620 nm on a plate reader "Uniplan". When adding hydrogen peroxide to the background of the calf blood gemodializat been decreasing apoptosis of these cells with 43 to 17% relative to apoptosis in the presence of a hydrogen peroxide. Under these conditions, the calf blood gemodializat significantly reduced ROS formation in human neuroblastoma cells SK-N-SH by the action of hydrogen peroxide. In these cells, we investigated the influence of calf blood gemodializat on apoptosis and intracellular signaling pathway involving mitogen-activated protein kinase (p38MAPK), extracellular regulatory kinase (ERK), phosphatidylinositol 3-kinase (PI-3K) and-Jun-N-terminal kinase (JNK) using their selective inhibitors. It was shown that the mechanism of the protective effect of calf blood gemodializat against peroxide-induced apoptosis in SK-N-SH dominant role is played by p38 MAPK and PI-3K.