Study of Rhizoma Darynaria on regulation of cellular immune function in immunosuppressive mice

• Published in: Wei sheng yan jiu Vol. 46; no. 1; p. 84
• Main Authors: Zhao, Gan, He, Guanlan, Lu, Chunyuan, Lao, Lingling, Wei, Jinbin
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2017
• Subjects: cellular immune; immunosuppression; regulation; Rhizoma drynaria ethanol extract
• ISSN: 1000-8020

To study the effect of Rhizoma Drynaria ethanol extract on regulation of cellular immune functions in cyclophosphamide-induced immunosuppressive mice. A total of 60 kunming mice were randomly divided into six groups:normal control group, model group, positive control group( levamisole, 0. 05 g / kg) and low-, medium- and high-dose Rhizoma Drynaria ethanol extract-treated groups( 2. 5, 5, 10 g / kg). The mice in the four administration group were administered with 10 mL / kg of the drug for 20 days. Except normal control group, the mice in the other 5 groups were intraperitoneally injected( ip) with cyclophosphamide to establish immunosuppression model. After the end of the experiment, the indices of lymphocyte proliferation capacity and delayed type hypersensitivity were detected, speen histological structure and Tlymphocyte subsets CD_4~+, CD_8~+T cells in peripheral blood were measured. The relativemRNA expression of Interleukin 4( IL-4) and Interferon-γ( IFN-γ) in the spleen tissues were detected. Rhizoma