Histone acetylation regulates osteodifferentiation of human dental pulp stem cells via DSPP

Dental pulp stem cells (DPSCs) are a unique population of precursor cells isolated from postnatal human dental pulp, with the ability to regenerate a reparative dentin-like complex. We examined the regulation of odontoblast-like differentiation of DPSCs by histone acetylation. Western blot analysis...

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Bibliographic Details
Published inFrontiers in bioscience (Landmark. Print) Vol. 18; no. 3; p. 1072
Main Authors Gu, Shensheng, Liang, Jingping, Wang, Jia, Liu, Bin
Format Journal Article
LanguageEnglish
Published Singapore 01.06.2013
Subjects
Acetylation
Adult
Cell Differentiation
Cells, Cultured
Dental Pulp - cytology
Histones - metabolism
Humans
Stem Cells - cytology
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Summary:Dental pulp stem cells (DPSCs) are a unique population of precursor cells isolated from postnatal human dental pulp, with the ability to regenerate a reparative dentin-like complex. We examined the regulation of odontoblast-like differentiation of DPSCs by histone acetylation. Western blot analysis showed that histone H3 acetylation was strongly induced in osteodifferentiation medium. Inhibition of histone acetyltransferase by garcinol reversed osteodifferentiation and mineral formation. Real-time polymerase chain reaction assay indicated that the dentin sialophosphoprotein (DSPP) gene, which is mainly expressed in odontoblasts and preameloblasts in teeth and plays an important role in tooth function, was also down-regulated in garcinol-treated cells. Moreover, lentivirus-mediated knockdown of DSPP in human DPSCs was associated with significant inhibition of mineral formation, but not osteoblast differentiation. In conclusion, the results of this study suggest that DSPP positively affects mineral formation, and that odontoblast-like differentiation and maturation of DPSCs can be regulated by histone acetylation of the DSPP gene.
ISSN:2768-6698