CD23 on B cells determines Breg-facilitated IL-10 secretion as well as activation of T cells

• Published in: Lin chuang er bi yan hou ke za zhi Vol. 32; no. 12; p. 931
• Main Authors: Chen, Q, Ba, Y P, Zhou, M H, Li, S D, Zhang, P W
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2018
• Subjects: Allergens; Animals; B-Lymphocytes - metabolism; Interleukin-10 - metabolism; Nasal Mucosa; Rats; Rhinitis, Allergic - metabolism; rhinitis，allergic; CD23; Bregs; IL-10
• ISSN: 1001-1781
• DOI: 10.13201/j.issn.1001-1781.2018.12.012

To explore whether or not the IL-10 mediated by Bregs modulate the secreting T cells activation by the anti-CD23 antibody, to find a new target for the treatment of allergic rhinitis. The rat model of allergic rhinitis was established. Anti-CD23 antibody was used to observe the behavioral changes, passive skin allergen test, enzyme-linked immunosorbent assay, hematoxylin and eosin staining, immunofluorescence and flow cytometry serological indicators, systemic and nasal mucosa. Compared with the blank control group, allergic rhinitis group rats sneezing, flexible nose, runny nose, subcutaneous mass increases;The levels of IL-10, IFN-γ and Bregs in blood decreased, the levels of IL-4, CD23+ B cells and CD4+ T cells increased;Nasal mucosa CD23 fluorescence intensity increased, CD19 and IL-10 fluorescence intensity decreased. Compared with the allergic rhinitis group, the number of sneezing, the frequency of nasal flexion, the symptoms of runny nose and the subcutaneous mass in the antibody intervention group we