Role and mechanism of platelet-derived growth factor BB in thrombocytosis in Kawasaki disease

• Published in: Zhongguo dang dai er ke za zhi Vol. 25; no. 6; pp. 579 - 586
• Main Authors: Shen, Xi-Wei, Tang, Zhi-Yuan, Shen, Xian-Juan, Zhao, Jian-Mei
• Format: Journal Article
• Language: Chinese
• Published: China 15.06.2023
• Subjects: Animals; Becaplermin; Child; Humans; Imatinib Mesylate - pharmacology; Imatinib Mesylate - therapeutic use; Mice; Mice, Inbred C57BL; Mucocutaneous Lymph Node Syndrome - drug therapy; Phosphatidylinositol 3-Kinases; Proto-Oncogene Proteins c-akt; RNA, Messenger; Thrombocytosis - drug therapy; Thrombocytosis - etiology; Thrombocytosis; Kawasaki disease; Mouse; Dami cell; Child; Platelet-derived growth factor BB
• ISSN: 1008-8830
• DOI: 10.7499/j.issn.1008-8830.2301086

To study the role and mechanism of platelet-derived growth factor BB (PDGF-BB) on platelet production in Kawasaki disease (KD) mice and human megakaryocytic Dami cells through and experiments. ELISA was used to measure the expression of PDGF in the serum of 40 children with KD and 40 healthy children. C57BL/6 mice were used to establish a model of KD and were then randomly divided into a normal group, a KD group, and an imatinib group (30 mice in each group). Routine blood test was performed for each group, and the expression of PDGF-BB, megakaryocyte colony forming unit (CFU-MK), and the megakaryocyte marker CD41 were measured. CCK-8, flow cytometry, quantitative real-time PCR, and Western blot were used to analyze the role and mechanism of PDGF-BB in platelet production in Dami cells. PDGF-BB was highly expressed in the serum of KD children ( <0.001). The KD group had a higher expression level of PDGF-BB in serum ( <0.05) and significant increases in the expression of CFU-MK and CD41 ( <0.001), and the imat