Study on biomarkers of acteoside in treating puromycin aminonucleoside nephropathy in young rats based on non-targeted urine metabolomics technology

This study aims to reveal the endogenous metabolic characteristics of acteoside in the young rat model of purinomycin aminonucleoside nephropathy(PAN) by non-targeted urine metabolomics and decipher the potential mechanism of action. Biochemical indicators in the urine of rats from each group were d...

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Published inZhongguo zhongyao zazhi Vol. 48; no. 21; p. 5898
Main Authors Wang, Meng-Xiao, Luo, Ke-Ke, Gao, Wen-Ya, Tian, Meng-Yao, Zhao, Hai-Yu, Si, Nan, Bian, Bao-Lin, Wei, Xiao-Lu, Wang, Hong-Jie, Zhou, Yan-Yan
Format Journal Article
LanguageChinese
Published China 01.11.2023
Subjects
Acetophenones
Amino Acids
Animals
Biomarkers - urine
Child
Chromatography, High Pressure Liquid - methods
Glomerulonephritis
Humans
Metabolomics - methods
Phenylalanine
Puromycin Aminonucleoside
Rats
acteoside
purinomycin aminonucleoside nephropathy
biomarker
non-targeted urine metabonomics
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Summary:This study aims to reveal the endogenous metabolic characteristics of acteoside in the young rat model of purinomycin aminonucleoside nephropathy(PAN) by non-targeted urine metabolomics and decipher the potential mechanism of action. Biochemical indicators in the urine of rats from each group were determined by an automatic biochemical analyzer. The potential biomarkers and related core metabolic pathways were identified by ultra-high performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry(UHPLC-LTQ-Orbitrap MS) combined with principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA). MetaboAnalyst 5.0 was used to establish the receiver operating characteristic(ROC) curve for evaluating the clinical diagnostic performance of core metabolites. The results showed that acteoside significantly decreased urinary protein-to-creatinine ratio in PAN young rats. A total of 17 differential metabolites were screened out by non-targeted urine me
ISSN:1001-5302
DOI:10.19540/j.cnki.cjcmm.20230707.406