Etiologic characteristics of Shigella sonnei strains isolated from some areas of Guangdong province and Guangxi Zhuang Autonomous Region of China, 2014-2016

• Published in: Zhōnghuá liúxíngbìng zázhì Vol. 38; no. 11; p. 1541
• Main Authors: Li, B S, Chen, L J, Ke, B X, Lin, J M, Xu, L Q, Tan, H L, He, D M, Liang, Y H, Ke, C W, Zhang, Y H
• Format: Journal Article
• Language: Chinese
• Published: China 10.11.2017
• Subjects: Anti-Bacterial Agents - pharmacology; Anti-Infective Agents - pharmacology; China; Disease Outbreaks; Drug Resistance, Bacterial; Dysentery, Bacillary - microbiology; Electrophoresis, Gel, Pulsed-Field; Humans; Microbial Sensitivity Tests; Sequence Analysis, DNA; Shigella sonnei - classification; Shigella sonnei - drug effects; Shigella sonnei - genetics; Shigella sonnei - isolation & purification; Tetracycline - pharmacology; Shigella sonnei; Pulsed-field gel electrophoresis; Whole genome sequencing; Etiology characteristic
• ISSN: 0254-6450
• DOI: 10.3760/cma.j.issn.0254-6450.2017.11.021

To investigated the etiologic characteristics of strains causing outbreaks and sporadic cases in some areas of Guangdong province and Guangxi Zhuang Autonomous Region during 2014-2016. Fourteen strains isolated from outbreaks and 6 strains from sporadic cases from Guangdong and Liuzhou of Guangxi Zhuang Autonomous Region were tested for antimicrobial resistance and analyzed by pulsed-field gel electrophoresis (PFGE). Six typical strains were selected for whole genome sequencing typing and compared with 51 strains isolated both at home and abroad from NCBI genome database. The antibiotic resistance test indicated the isolates had high resistance rate to ampicillin, tetracycline, gentamicin, trimethoprim/sulfamethoxazole and nalidixic acid, while sensitive to azithromycin, chloromycetin and imipenem. PFGE showed high similarity (93.2%) among the strains isolated from different areas. The whole genome sequencing analysis also revealed that all the typical strains were clustered into a same evolution branch, clos