The effect of PM(2.5) on oncogene expression in HBE cells

• Published in: Zhonghua laodong weisheng zhiyebing zazhi Vol. 37; no. 8; p. 561
• Main Authors: Zheng, K, Wang, B Y, Xu, X Y, Geng, H, Long, D X
• Format: Journal Article
• Language: Chinese
• Published: China 20.08.2019
• Subjects: Fine particulate matter (PM(2.5)); Human bronchial epithelial cells; Gene expression; Haze; Oncogenes
• ISSN: 1001-9391
• DOI: 10.3760/cma.j.issn.1001-9391.2019.08.001

To study the effect of particulate matter 2.5 (PM(2.5)) on oncogene expression in human bronchial epithelial (HBE) cells. HBE cells were selected as the study subjects, and PM(2.5) treatment group (10 μg/ml and 50 μg/ml) , negative control group and positive control group (10 μmol/L Cr(6+)) were set. CCK8 assay was used to test the IC(50) value of PM(2.5). HBE cells were treated with PM(2.5) for 24 h at 10 μg/ml and 50 μg/ml, additionally, cells were treated with blank as negative control, 10 μmol/L Cr(6+) as a positive control for 24 h. After the treatment, mRNA expression of oncogenes including c-myc, c-fos, k-ras and p53 were detected by fluorescent quantitative RT-PCR, the protein expression of oncogenes were detected with western blot. The IC(50) value of PM(2.5) in HBE cells is 70.12 μg/ml. The qRT-PCR data showed that compared with the control group, the expression level of c-myc gene increased by respectively 500.1%、780.7%、305.3% after exposure to 10、50 μg/ml PM(2.5) and positive control group; c-fos