Mechanism of astragaloside Ⅳ in regulating autophagy of PC12 cells under oxygen-glucose deprivation by medicating Akt/mTOR/HIF-1α pathway

• Published in: Zhongguo zhongyao zazhi Vol. 48; no. 19; p. 5271
• Main Authors: Long, Jia-Xin, Tian, Meng-Zhi, Chen, Xiao-Yi, Xiong, Yu, Yu, Huang-He, Gong, Yong-Zhen, Ding, Huang, Xie, Ming-Xia, DU, Ke
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2023
• Subjects: Animals; Apoptosis; Autophagy; Beclin-1 - genetics; Beclin-1 - pharmacology; Glucose - therapeutic use; Oxygen - metabolism; PC12 Cells; Proto-Oncogene Proteins c-akt - genetics; Rats; Reperfusion Injury - drug therapy; TOR Serine-Threonine Kinases - genetics; TOR Serine-Threonine Kinases - metabolism; astragaloside Ⅳ(AS-Ⅳ); autophagic injury; Akt/mTOR/HIF-1α; oxygen-glucose deprivation(OGD)
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20230630.402

This study explored the protective effect of astragaloside Ⅳ(AS-Ⅳ) on oxygen-glucose deprivation(OGD)-induced autophagic injury in PC12 cells and its underlying mechanism. An OGD-induced autophagic injury model in vitro was established in PC12 cells. The cells were divided into a normal group, an OGD group, low-, medium-, and high-dose AS-Ⅳ groups, and a positive drug dexmedetomidine(DEX) group. Cell viability was measured using the MTT assay. Transmission electron microscopy was used to observe autophagosomes and autolysosomes, and the MDC staining method was used to assess the fluorescence intensity of autophagosomes. Western blot was conducted to determine the relative expression levels of functional proteins LC3-Ⅱ/LC3-Ⅰ, Beclin1, p-Akt/Akt, p-mTOR/mTOR, and HIF-1α. Compared with the normal group, the OGD group exhibited a significant decrease in cell viability(P<0.01), an increase in autophagosomes(P<0.01), enhanced fluorescence intensity of autophagosomes(P<0.01), up-regulated Beclin1, LC3-Ⅱ/LC3-Ⅰ, and H