Determination of seven ingredients of different grades Alismatis Rhizoma by QAMS method

• Published in: Zhongguo zhongyao zazhi Vol. 44; no. 11; p. 2292
• Main Authors: Tai, Yan-Ni, Weng, Yan-Hong, Zhang, Su-Ping, Xu, Wen, Li, Xiao-Yan, Lin, Qing-Qing, Chu, Ke-Dan, Wu, Shui-Sheng
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2019
• Subjects: Alismatis Rhizoma; different grades; quantitative analysis of multi-components by single marker; sesquiterpenes
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20190321.205

The present study is to establish a quantitative analysis of multi-components by single marker(QAMS) for determining contents of seven compositions in Alismatis Rhizoma, alismoxide, alisol C 23-acetate, alisol A, alismol, alisol B, alisol B 23-acetate and 11-deoxy-alisol B. Six relative correction factors(RCFs) of alismoxide, alisol C 23-acetate, alisol A, alismol, alisol B and 11-deoxy-alisol B were established in the UPLC method with alisol B 23-acetate as the internal standard, which was to calculate the mass fraction of each. The mass fraction of seven effective constituents in Alismatis Rhizoma was calculated by the external standard method(ESM) at the same time. Compared with the content results determined by the ESM and QAMS, the feasibility and accuracy of QAMS method were verified. Within the linear range, the RCFs of alismoxide, alisol C 23-acetate, alisol A, alismol, alisol B, 11-deoxy-alisol B were 0.946, 4.183, 0.915, 1.039, 0.923 and 1.244, respectively, with good repeatability in different expe