Role of reactive oxygen species in hypoxia-induced non-small cell lung cancer migration

• Published in: Zhong hua yi xue za zhi Vol. 97; no. 40; p. 3174
• Main Authors: Wei, Y L, Dong, H M, Xie, Z F, Cai, S X
• Format: Journal Article
• Language: Chinese
• Published: China 31.10.2017
• Subjects: Carcinoma, Non-Small-Cell Lung - physiopathology; Cell Hypoxia; Cell Line, Tumor; Cell Movement; Humans; Hypoxia; Lung Neoplasms - physiopathology; Proto-Oncogene Proteins c-akt - metabolism; Reactive Oxygen Species; Carcinoma, non-small cell lung; Reactive oxygen species; Cell hypoxia; Neoplasm metastasis
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2017.40.012

To explore reactive oxygen species (ROS) generation and its role on A549 cell migration under hypoxic condition. Human non-small cell lung cancer (NSCLC) cell line A549 was incubated in a hypoxic environment (1%O(2), hypoxia group) or in a normoxic environment (21%O(2), normoxia group). The generation of ROS was measured by flow cytometry. The cell motility of A549 cells was detected by Transwell assay. The protein levels of protein kinase B (AKT), p38 mitogen-activated protein kinase (p38) were determined by Western blot analysis. After 16 h hypoxic treatment, the migration of A549 cells in hypoxia group was significantly more than that of normoxia group [(85±10) vs (56±7) per lower magnification, <0.001]. Besides, the generation of ROS was in a time-depended manner in hypoxia group. The ROS level was increased with the prolonged hypoxia time. It was significantly higher at 24 h than that in normoxia group [(273±4)% vs (102±6)%, <0.001]. The migrated cells in hypoxia group co-treated with 2 mmol/L NAC for 16