Protective effects of a wheat germ peptide (RVF) against H2O 2-induced oxidative stress in human neuroblastoma cells

• Published in: Biotechnology letters Vol. 36; no. 8; pp. 1615 - 1622
• Main Authors: Cheng, Yunhui, Zhang, Li, Sun, Wei, Tang, Jiqing, Lv, Zhaoliang, Xu, Zhou, Yu, Huixin
• Format: Journal Article
• Language: English
• Published: Netherlands 01.08.2014
• Subjects: Antioxidants - pharmacology; Apoptosis - drug effects; Calcium - metabolism; Caspase 3 - metabolism; Cell Line, Tumor; Cell Survival - drug effects; Cytoprotection - drug effects; Humans; Hydrogen Peroxide - toxicity; Membrane Potential, Mitochondrial - drug effects; Neuroblastoma - enzymology; Neuroblastoma - pathology; Neuroprotective Agents - pharmacology; Oligopeptides - pharmacology; Oxidative Stress - drug effects; Poly(ADP-ribose) Polymerases - metabolism
• ISSN: 1573-6776
• DOI: 10.1007/s10529-014-1521-6

RVF (Arg-Val-Phe), a peptide derived from wheat germ, shows antioxidant properties. Here, the neuroprotective efficacies of RVF were investigated in human neuroblastoma cells (SH-SY5Y) that were pretreated with RVF (150-250 μM, 4 h) and exposed to H2O2 (200 μM). RVF increased viable cell numbers by 37 % and reduced the release of lactate dehydrogenase. Pretreatment with RVF also inhibited H2O2-induced accumulation of reactive oxygen species and maintained the mitochondrial transmembrane potential as well as preventing intracellular Ca(2+) dysregulation during H2O2 exposure. Furthermore, pretreatment with RVF increased the Bcl-2/Bax ratio and blocked cleavage poly(ADP-ribose) polymerase by inhibiting caspase-3 activation, thus decreasing apoptosis.