Qualitative and quantitative analysis of alkaloids in Eurycoma longifolia by HPLC-Q-TOF-MS combined with UPLC-QQQ-MS/MS

A sensitive and efficient method was established and validated for qualitative and quantitative analysis of total alkaloids from the extract of Eurycoma longifolia by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(HPLC-Q-TOF-MS) combined with ultra-pe...

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Bibliographic Details
Published inZhongguo zhongyao zazhi Vol. 46; no. 24; p. 6435
Main Authors Chen, Xiu-Ming, Lin, Bin, Fang, Dong-Sheng
Format Journal Article
LanguageChinese
Published China 01.12.2021
Subjects
Alkaloids
Chromatography, High Pressure Liquid
Chromatography, Liquid
Eurycoma
Tandem Mass Spectrometry
HPLC-Q-TOF-MS
Eurycoma longifolia
UPLC-QQQ-MS/MS
alkaloid
quantitative analysis
qualitative analysis
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Summary:A sensitive and efficient method was established and validated for qualitative and quantitative analysis of total alkaloids from the extract of Eurycoma longifolia by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(HPLC-Q-TOF-MS) combined with ultra-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry(UPLC-QQQ-MS/MS). The HPLC-Q-TOF-MS conditions are as follows: Welch Ultimate XB-C_(18) column(4.6 mm×250 mm, 5 μm) with acetonitrile(containing 0.1% formic acid)-0.1% formic acid in water as mobile phase for gradient elution. The UPLC-QQQ-MS/MS conditions are as below: Agilent Eclipse Plus C_(18) column(2.1 mm×50 mm, 1.8 μm) with acetonitrile(containing 0.1% formic acid) and 0.1% formic acid in water as mobile phase for gradient elution. MS data were collected by electrospray ionization in positive ion mode. According to the comparison with reference standards and the accurate masses of molecules, a total of 17 alkaloids in E. l
ISSN:1001-5302
DOI:10.19540/j.cnki.cjcmm.20210802.202