Bipolar Action of Inhibitor of Vasculogenic Mimicry on Gene Expression in Melanoma Cells

• Published in: Molekuliarnaia biologiia Vol. 58; no. 2; p. 295
• Main Authors: Tchurikov, N A, Vartanian, A A, Klushevskaya, E S, Alembekov, I R, Kretova, A N, Chechetkin, V R, Kravatskaya, G I, Kosorukov, V S, Kravatsky, Y V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.03.2024
• Subjects: Cell Cycle - drug effects; Cell Line, Tumor; Gene Expression Regulation, Neoplastic - drug effects; Humans; Melanoma - drug therapy; Melanoma - genetics; Melanoma - metabolism; Melanoma - pathology; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; Neovascularization, Pathologic - drug therapy; Neovascularization, Pathologic - genetics; Neovascularization, Pathologic - metabolism; Neovascularization, Pathologic - pathology; Transcription Factors - genetics; Transcription Factors - metabolism; ACER2; LCS1269 inhibitor; TCAF2; KIF18A; NAV3; RNA-Seq; transcription factors; Matrigel; coordinated expression; melanoma; FGF13; NCKAP5
• ISSN: 0026-8984
• DOI: 10.31857/S0026898424020116,EDN:NIAKKQ

Multiple exogenous or endogenous factors alter gene expression patterns by different mechanisms that are poorly understood. We used RNA-Seq analysis in order to study changes in gene expression in melanoma cells that are capable of vasculogenic mimicry that is inhibited upon the action of an inhibitor of vasculogenic mimicry. Here, we show that the drug induces a strong upregulation of 50 genes that control the cell cycle and microtubule cytoskeleton coupled with a strong downregulation of 50 genes that control different cellular metabolic processes. We found that both groups of genes are simultaneously regulated by multiple sets of transcription factors. We conclude that one way for coordinated regulation of large groups of genes is regulation simultaneously by multiple transcription factors.