Measuring the rate constants of Ca²⁺ indicators
Fluorescent Ca(2+) indicators are widely used to measure the concentration of free Ca(2+) ([Ca(2+)](free)) in biological processes. To determine the exact kinetics of changes in [Ca(2+)](free) and the processes underlying these changes (e.g., Ca(2+) binding to Ca(2+)-binding proteins), it is necessa...
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Published in | Cold Spring Harbor protocols Vol. 2014; no. 7; p. 763 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
01.07.2014
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Subjects | |
Online Access | Get more information |
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Summary: | Fluorescent Ca(2+) indicators are widely used to measure the concentration of free Ca(2+) ([Ca(2+)](free)) in biological processes. To determine the exact kinetics of changes in [Ca(2+)](free) and the processes underlying these changes (e.g., Ca(2+) binding to Ca(2+)-binding proteins), it is necessary to know the exact binding properties of the indicator used. Here, we describe how to determine the binding rate constants (k(on) and k(off)) of Ca(2+) indicators. |
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ISSN: | 1559-6095 |
DOI: | 10.1101/pdb.prot073288 |