Impact of Liparis nervosa extract on neuroinflammation mediated by LPS-induced BV-2 microglial cells and its bioactive components analysis

• Published in: Zhongguo zhongyao zazhi Vol. 49; no. 11; p. 3050
• Main Authors: Kong, Shuai-Wen, Zhang, Yuan, Zeng, Lian, Chen, Xi-Rong, Wang, Hai-Jing, Cai, He-Lun, Hao, Xin-Cai, Zhang, Yu
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2024
• Subjects: Animals; Cell Line; Cell Survival - drug effects; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - pharmacology; Interleukin-6 - genetics; Interleukin-6 - metabolism; Lipopolysaccharides; Mice; Microglia - drug effects; Microglia - metabolism; Neuroinflammatory Diseases - drug therapy; Nitric Oxide - metabolism; Plant Extracts - chemistry; Plant Extracts - pharmacology; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism; Liparis nervosa; microglial cells; neuroinflammation; MAPK signaling pathway; NF-κB signaling pathway; chemical component
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20240122.701

To investigate the impact and potential mechanisms of extracts from different parts of Liparis nervosa on neuroinflammation by lipopolysaccharide(LPS)-induced BV-2 microglial cells. The materials of L. nervosa were subjected to crushing, ethanol extraction, and concentration to obtain an alcohol extract. Subsequently, the extract was further extracted to obtain petroleum ether extract, ethyl acetate extract, N-butanol extract, and aqueous phase extract. The ethyl acetate extract was separated into distillate(1)-(6)using D101 macroporous resin column chromatography. The experiment was divided into control group, LPS model group, L. nervosa extract group, and LPS + L. nervosa group. LPS was utilized to induce a neuroinflammatory cell model in BV-2 microglial cells. The Griess test was utilized for detecting the production of nitric oxide(NO) in the cell supernatant. Cell viability was detected by MTT assay. The release of interleukin-6(IL-6) and tumor necrosis factor alpha(TNF-α) in the cell supernatant was qua