Screening and validation of RT-qPCR reference genes of Viola yedoensis under different light intensities

• Published in: Zhongguo zhongyao zazhi Vol. 46; no. 21; p. 5620
• Main Authors: Yang, Pei-Fang, Shi, Hong-Zhuan, Guo, Qiao-Sheng, Zhao, Kun, Liu, Li
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2021
• Subjects: Gene Expression Profiling; Real-Time Polymerase Chain Reaction; Reference Standards; Viola; reference gene; Ge Norm; RT-qPCR; Viola yedoensis; Norm Finder
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20210816.103

Screening the reference genes that were stably expressed under different light intensities for Viola yedoensis could provide reference for the related molecular research. In this study, 11 candidate reference genes were detected by RT-qPCR for tissues of V. yedoensis treated with different light intensities. Ge Norm, Norm Finder, Best Keeper, and Ref Finder website were used to comprehensively evaluate the reference genes, and verify the stability of the reference gene based on CAT1. Finally, the ideal reference gene was determined. The results showed that CYP, Actin, and SAMDC had small Ct value ranges and stable expression. Ge Norm demonstrated that CYP, SAMDC, and Actin were suitable reference genes. Norm Finder showed that the expression of α-TUB was the most stable. Best Keeper recommended CYP, Actin, and SAMDC as reference genes. Ref Finder assessed that SAMDC, CYP, α-TUB, and Actin had better stability, while GAPDH had the worst stability. The expression trend of CAT1 gene was consistent when calibrate