Determination of sixteen antibiotics and four β -agonists in human urine samples using ultra-performance liquid chromatography-tandem mass spectrometry based on high-throughput automatic solid-phase extraction

• Published in: Se pu = Chinese journal of chromatography Vol. 41; no. 5; p. 397
• Main Authors: Li, Zhen-Huan, Hu, Xiao-Jian, Lu, Yi-Fu, Xie, Lin-Na, Zhu, Ying
• Format: Journal Article
• Language: Chinese
• Published: China 08.05.2023
• Subjects: Albuterol; Anti-Bacterial Agents - analysis; Chromatography, High Pressure Liquid; Chromatography, Liquid; Clenbuterol; Humans; Methanol; Solid Phase Extraction; Tandem Mass Spectrometry - methods; Terbutaline; Water; ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS); agonists; urine; antibiotics; solid-phase extraction (SPE)
• ISSN: 1872-2059
• DOI: 10.3724/SP.J.1123.2022.08025

An analytical method combining high-throughput automatic solid-phase extraction with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to determine 16 antibiotics (macrolides, tetracyclines, quinolones, and sulfonamides) and 4 -agonists (terbutaline, salbutamol, ractopamine, and clenbuterol) in human urine samples. After thawing at room temperature, 1 mL of urine was sampled and the internal standard was added, followed by the addition of 200 μL ammonium acetate buffer and 20 μL -glucuronidase, and the mixture was incubated at 37 ℃ overnight. Automatic solid-phase extraction was used to extract the target compounds from the urine samples, and the recoveries were compared using different solid-phase extraction 96-well plates (PRiME MCX, Sep-Pak C , PRiME HLB), types and volumes of rinse solutions and eluents. Satisfactory recoveries of the 20 target compounds were obtained using the Oasis PRiME HLB 96-well plate, with 1.5 mL 10% (v/v) methanol aqueous solution and 2.0