Research progress of visual detection in rapid on-site detection of pathogen nucleic acid

• Published in: Yíchuán Vol. 45; no. 4; p. 306
• Main Authors: Liu, Dan-Ni, Wu, Hai-Ping, Zhou, Guo-Hua
• Format: Journal Article
• Language: English
• Published: China 20.04.2023
• Subjects: CRISPR-Cas Systems; Nucleic Acid Amplification Techniques - methods; Nucleic Acids; Technology; point-of-care testing; CRISPR/Cas system; visual detection; pathogen detection; isothermal amplification
• ISSN: 0253-9772
• DOI: 10.16288/j.yczz.22-323

Nucleic acid detection is widely used in pathogen screening and detection due to its high sensitivity and specificity. With the increase of detection requirements and the development of amplification technology, nucleic acid detection methods are gradually developing towards simple, fast and low-cost. Quantitative polymerase chain reaction (qPCR), as the "gold standard" for nucleic acid detection, relies on expensive equipment and professional operators, which is not suitable for rapid on-site detection of pathogens. The visual detection method without relying on excitation light source or complex equipment can present the detection results in a more intuitive and portable way after combining with rapid and efficient amplification technology, which has the potential of point-of-care testing (POCT). This paper focuses on the reported application of amplification technology and CRISPR/Cas technology in visual detection and compares their advantages and disadvantages, so as to provide reference for POCT strategy based on pathogen nucleic acid.