Changes in secretory pathway Ca(2+)-ATPase 2 following focal cerebral ischemia/reperfusion injury

• Published in: Neural regeneration research Vol. 8; no. 1; pp. 76 - 82
• Main Authors: Lu, Tonglin, Hu, Zhiping, Zeng, Liuwang, Jiang, Zheng
• Format: Journal Article
• Language: English
• Published: India 05.01.2013
• Subjects: photographs-containing paper; oxidative damage; Ca2+-ATPase; manganese; Golgi apparatus; brain injury; neural regeneration; cerebral infarction; neuroregeneration; grants-supported paper; secretory pathway Ca2+-ATPase 2; Ca2+ oscillations; focal cerebral ischemia
• ISSN: 1673-5374
• DOI: 10.3969/j.issn.1673-5374.2013.01.010

This study aimed to investigate changes in secretory pathway Ca(2+)-ATPase 2 expression following cerebral ischemia/reperfusion injury, and to define the role of Ca(2+)-ATPases in oxidative stress. A rat model of cerebral ischemia/reperfusion injury was established using the unilateral middle cerebral artery occlusion method. Immunohistochemistry and reverse transcription-PCR assay results showed that compared with the control group, the expression of secretory pathway Ca(2+)-ATPase 2 protein and mRNA in the cerebral cortex and hippocampus of male rats did not significantly change during the ischemic period. However, secretory pathway Ca(2+)-ATPase 2 protein and mRNA expression reduced gradually at 1, 3, and 24 hours during the reperfusion period. Our experimental findings indicate that levels of secretory pathway Ca(2+)-ATPase 2 protein and mRNA expression in brain tissue change in response to cerebral ischemia/reperfusion injury.