Analysis of related factors influencing the detection rate of mosaic embryo and the pregnancy outcomes with mosaic embryo transfers

• Published in: Chung-hua fu chʿan kʿo tsa chih Vol. 59; no. 4; p. 288
• Main Authors: Zhang, Q, Xiong, S, Han, W, Liu, D Y, Huang, G N, Lin, T T
• Format: Journal Article
• Language: Chinese
• Published: China 25.04.2024
• Subjects: Adult; Aneuploidy; Blastocyst - cytology; Embryo Transfer - methods; Female; Fertilization in Vitro; Genetic Testing - methods; High-Throughput Nucleotide Sequencing; Humans; Live Birth; Mosaicism; Pregnancy; Pregnancy Outcome; Pregnancy Rate; Preimplantation Diagnosis - methods; Retrospective Studies
• ISSN: 0529-567X
• DOI: 10.3760/cma.j.cn112141-20240104-00010

To explore the related factors influencing the detection rate of mosaic embryo and the pregnancy outcomes of mosaic embryo transfer in preimplantation genetic testing for aneuploidy (PGT-A) based on next generation sequencing (NGS) technology. A retrospective study was performed to analyze the clinical data of patients in 745 PGT-A cycles from January 2019 to May 2023 at Chongqing Health Center for Women and Children, including 2 850 blastocysts. The biopsy cells were tested using NGS technology, and the embryos were divided into three groups based on the test results, namely euploid embryos, aneuploid embryos and mosaic embryos. The influence of population characteristics and laboratory-related parameters on the detection rate of mosaic embryo were analyzed, and the pregnancy outcomes of 98 mosaic embryo transfer cycles and 486 euploid embryo transfer cycles were compared during the same period, including clinical pregnancy rate and live birth rate. Among the embryos tested ( =2 850), the number and proporti