Effect of Different Reclaimed Water Irrigation Methods on Bacterial Community Diversity and Pathogen Abundance in the Soil-Pepper Ecosystem

• Published in: Huanjing kexue Vol. 40; no. 11; p. 5151
• Main Authors: Cui, Bing-Jian, Gao, Feng, Hu, Chao, Li, Zhong-Yang, Fan, Xiang-Yang, Cui, Er-Ping
• Format: Journal Article
• Language: Chinese
• Published: China 08.11.2019
• Subjects: Agricultural Irrigation; Bacteria; Rhizosphere; Soil; Soil Microbiology; Water; bacterial diversity; reclaimed water irrigation; pathogens; high-throughput sequencing; real-time quantitative PCR
• ISSN: 0250-3301
• DOI: 10.13227/j.hjkx.201904269

Reclaimed water is considered to be a reasonable and sustainable alternative water resource to improve water resource layout and mitigate the shortage of traditional water resources. Its use in irrigation will cause changes in the microbial community structure and opportunistic pathogen abundance in soils and crops, but few studies have been conducted on this subject. Peppers were used as the research subjects, and the treatments were direct irrigation of reclaimed water, mixed irrigation with freshwater and reclaimed water, rotated irrigation with freshwater and reclaimed water, with potable water irrigation as the control. The effects of different irrigation methods of reclaimed water on the soil physicochemical properties were analyzed through a pot experiment. Furthermore, changes in bacterial community and opportunistic pathogen abundance in pepper fruit and the rhizosphere under reclaimed water irrigation conditions were investigated based on high-throughput sequencing technology and quantitative PCR me