Purification of African Swine Fever Virus

African swine fever virus is a cytolytic virus that leads to the apoptosis of both cultured cells and primary macrophages. Cell culture supernatants of virus-infected cells are routinely used for virological and immunological studies, despite differences in the biological behavior between such prepa...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2503; p. 179
Main Authors Shimmon, Gareth L, Shah, Pranav N M, Fry, Elizabeth, Stuart, David I, Hawes, Pippa, Netherton, Christopher L
Format Journal Article
LanguageEnglish
Published United States 2022
Subjects
African Swine Fever
African Swine Fever Virus
Animals
Cell Line
Cells, Cultured
DNA Viruses
Swine
Viral Proteins
Percoll
African swine fever virus
Purification
Vero cells
Density centrifugation
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Summary:African swine fever virus is a cytolytic virus that leads to the apoptosis of both cultured cells and primary macrophages. Cell culture supernatants of virus-infected cells are routinely used for virological and immunological studies, despite differences in the biological behavior between such preparations and highly purified virus. In addition, more recent data suggests that exosomes containing viral proteins may be secreted from infected cells. While African swine fever virus can be purified through a number of methods, in our hands Percoll provides the most robust method of separating virus from cellular contaminants.
ISSN:1940-6029
DOI:10.1007/978-1-0716-2333-6_13