Effects of Huangqin Tang on NLRP3/Caspase-1 pathway in mice model of ulcerative colitis

• Published in: Zhongguo zhongyao zazhi Vol. 48; no. 1; p. 226
• Main Authors: Liu, Meng-Ru, Li, Hui, Wei, Lan-Fu, Liu, Xiao-Tong, An, Zhen-Tao, Gu, Li-Mei, Tian, Yao-Zhou
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2023
• Subjects: Animals; Caspase 1 - genetics; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - drug therapy; Colitis, Ulcerative - genetics; Colon; Dextran Sulfate - adverse effects; Disease Models, Animal; Drugs, Chinese Herbal - pharmacology; Interleukin-10 - genetics; Interleukin-6 - genetics; Mesalamine - pharmacology; Mice; Mice, Inbred C57BL; NLR Family, Pyrin Domain-Containing 3 Protein - genetics; Scutellaria baicalensis - chemistry; Tumor Necrosis Factor-alpha - metabolism; pyroptosis; Huangqin Tang(HQT); NLRP3/Caspase-1 signaling pathway; ulcerative colitis(UC)
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20221018.502

The aim of this study was to explore the effects of Huangqin Tang(HQT) on the NLRP3/Caspase-1 signaling pathway in mice with DSS-induced ulcerative colitis(UC). C57BL/6J mice were randomly divided into a blank group, a model group(DSS group), and low-, medium-and high-dose HQT groups(HQT-L, HQT-M, and HQT-H), and western medicine mesalazine group(western medicine group). The UC model was induced in mice. Subsequently, the mice in the HQT-L, HQT-M, HQT-H groups, and the western medicine group were given low-, medium-, high-dose HQT, and mesalazine suspension by gavage, respectively, while those in the blank and DSS groups were given an equal volume of distilled water by gavage. After 10 days of administration, the body weight, DAI scores, and colonic histopathological score of mice in each group were determined. The levels of IL-6, IL-10, IL-1β, and TNF-α in serum were determined by ELISA. The mRNA expression of NLRP3 and Caspase-1 in colon tissues was determined by RT-qPCR. The protein expression of NLRP3 and