Tanshinone ⅡA Ameliorates Cartilage Degeneration in Ovariectomized Rats by Regulating TGF-β1/Smad2/MMPs Signaling Pathway

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 55; no. 4; p. 878
• Main Authors: Guo, Qin, Guo, Yuanli, Liao, Feng'er, Tao, Ying
• Format: Journal Article
• Language: Chinese
• Published: China 20.07.2024
• Subjects: Abietanes - pharmacology; Abietanes - therapeutic use; Animals; Cartilage, Articular - drug effects; Cartilage, Articular - metabolism; Cartilage, Articular - pathology; Collagen Type II - metabolism; Female; Matrix Metalloproteinase 13 - metabolism; Matrix Metalloproteinase 9 - metabolism; Ovariectomy; Rats; Rats, Sprague-Dawley; Signal Transduction - drug effects; Smad2 Protein - metabolism; Transforming Growth Factor beta1 - metabolism; Articular cartilage; Ovariectomy; Rat; Tanshinone ⅡA
• ISSN: 1672-173X
• DOI: 10.12182/20240760204

To investigate the ameliorative effect of tanshinone ⅡA (Tan) on osteoarticular degeneration in ovariectomized rats (a postmenopausal estrogen deficiency model) and the mechanisms involved. Eight-week-old female Sprague Dawley (SD) rats were randomly allocated to 5 groups ( =10 each), including a Sham operation group (Sham), an ovariectomy group (OVX), and low, medium, and high-dose Tan groups. Eight weeks after bilateral ovariectomy, the rats in the low, medium, and high-dose Tan groups were treated with Tan at the doses of 5, 10, and 20 mg/kg for a duration of 28 days. Evaluation of the rat articular cartilage was performed using X-ray imaging, anatomical observation, hematoxylin and eosin (H&E) staining, and toluidine blue staining. Immunohistochemistry was performed to assess the expression levels of transforming growth factor β1 (TGF-β1), phosphorylated-smad2 (p-Smad2), type Ⅱ collagen (CⅡ), matrix metalloproteinase 9 (MMP-9), and MMP-13 in the cartilage tissue. The knee joints of the OVX rats exhibited