Effects of silence information regulator 7 on proliferation and apoptosis of mouse renal podocytes under high glucose environment

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 38; no. 6; p. 611
• Main Authors: Feng, Min, Lin, Ting, Chen, Xia-Xia, Yang, Xiao-Ling, Lyu, Qi, Wen, Jun-Ping
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2022
• Subjects: Animals; Apoptosis; beta Catenin; Cell Proliferation; Glucose; Mice; Podocytes; RNA, Small Interfering; Sincalide; apoptosis; SIRT7; renal podocytes; Wnt/β-catenin signaling pathway; high glucose; mice
• ISSN: 1000-6834
• DOI: 10.12047/j.cjap.6366.2022.111

To investigate the effects and its mechanisms of silence information regulator 7(SIRT7)on mouse renal podocytes proliferation and apoptosis under high glucose environment. Mouse renal podocytes cultured with high glucose and treated with different methods were divided into the following groups:control group(Control),high glucose group(HG),high glucose+transfecting with SIRT7 overexpression vetor(pcDNA3.1-SIRT7) group(SIRT7 OE+HG),high glucose+transfecting with the negative control vetor(pcDNA3.1)group(SIRT7 OE-NC+HG),high glucose+transfecting with small interfering RNA-SIRT7 (siRNA-SIRT7) group (siRNA-SIRT7+HG), high glucose+ transfecting with siRNA-SIRT7 control group (siRNA-SIRT7-NC+ HG). Viability of proliferation was examined by CCK-8 method.Rate of apoptosis was detected by flow cytometry. The level of SIRT7 mRNA expression was measured by qRT-PCR. Western blot was performed to detect the protein expression of Nephrin and key factors of Wnt/β-catenin signaling pathway. The CCK-8 result showed that,compar