Discovery of Culex inatomii (Diptera : Culicidae) in Chongming, Shanghai

• Published in: Zhongguo jishengchongxue yu jishengchongping zazhi Vol. 35; no. 1; p. 31
• Main Authors: Fang, Yuan, Shi, Wen-qi, Zhang, Yi, Hu, Qiu-an, Zhou, Zheng-bin, Wu, Jia-tong, Zhang, Liang-liang
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2017
• ISSN: 1000-7423

To record the discovery of Culex inatomii in Chongming, Shanghai. Larvae and adult mosquitos of Cx. inatomii were collected in Dongtan of Chongming Island from May to November in 2015 and 2016, and their morphological characteristics were observed. The genomic DNA was extracted from adult mosquitos, PCR was performed to amplify the cytochrome c oxidaseⅠ(COⅠ) gene. Multiple alignment of COⅠ sequence was conducted with ClustalW2. Pairwise distances within and between species were calculated using MEGA v5.10 based on COⅠ sequences of Cx. inatomii, Cx. modestus, Cx. pipiens pallens, and Cx. tritaeniorhynchus. The phylogenetic tree of the above four species was constructed using neighbor joining, maximum likelihood, and Bayesian methods. One hundred and fifty-six adult female mosquitos and 36 larvae of Cx. inatomii were collected. Larvae were reared to adult stage in the laboratory (17 female, 19 male). Morphologically, the subapical lobe of the sidepiece in male genitalia was divided into two parts, the anterior