Prion-like determinant [NSI+decreases expression of the SUP45 gene in Saccharomyces cerevisiae
Previously, we described and characterized yeast non-chromosomal determinant [NSI+], possessing prion properties. This determinant causes a decrease in translation termination fidelity, which is phenotypically detectable as the nonsense suppression in the strains with decreased functional activity o...
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Published in | Molekuliarnaia biologiia Vol. 48; no. 5; p. 790 |
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Main Authors | , , , |
Format | Journal Article |
Language | Russian |
Published |
Russia (Federation)
01.09.2014
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Subjects | |
Online Access | Get more information |
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Summary: | Previously, we described and characterized yeast non-chromosomal determinant [NSI+], possessing prion properties. This determinant causes a decrease in translation termination fidelity, which is phenotypically detectable as the nonsense suppression in the strains with decreased functional activity of eRF3 release factor. As a result of geneticscreen, we demonstrated that an increase in the expression of SUP45 encoding the eRF1 release factor (Sup45), masks, but does not eliminate nonsense suppression in the [NSI+] strains. In the present study, we first demonstrated the direct cause for the nonsense suppression in [NSI+] strains. We demonstrated that [NSI+] decreases the relative amounts of SUP45 mRNA that causes a decrease in the amounts of Sup45 protein that is detectable in the stationary growth phase. The data obtained suggest the structural protein of [NSI+] seems to be either a transcription factor or participates in the regulation of cellular mRNA stability. |
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ISSN: | 0026-8984 |