Analysis of enterovirus infection type among acute respiratory tract infection cases in Luohe City, Henan Province from 2017 to 2021

• Published in: Zhōnghuá yùfáng-yīxué zázhì Vol. 57; no. 3; p. 378
• Main Authors: Li, W X, Xie, Z B, Xu, J, Xia, B C, Duan, H J, Song, J H, Wang, H L, Xu, W W, Zhang, Y, Fan, H
• Format: Journal Article
• Language: Chinese
• Published: China 06.03.2023
• Subjects: 5' Untranslated Regions; Antigens, Viral - genetics; Enterovirus - genetics; Enterovirus Infections - epidemiology; Female; Humans; Male; Phenotype; Phylogeny; Respiratory Tract Infections - epidemiology
• ISSN: 0253-9624
• DOI: 10.3760/cma.j.cn112150-20221011-00985

To understand the infection status of Enterovirus (EV) in cases of acute respiratory infections (ARIs) in Luohe City, Henan Province from 2017 to 2021, and analyze the prevalence and type composition of EV in ARIs. From October 2017 to May 2021, pharyngeal swab samples were collected from 1 828 patients with ARIs in Luohe Central Hospital and the clinical epidemiological data of these cases were also collected. EV-positive samples were identified by Quantitative Real-time Polymerase Chain Reaction (qPCR). The 5'-untranslated region (5'UTR) was amplified by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The results of 5'UTR region were initially typed by Enterovirus Genotyping Tool Version 1.0. Based on the typing results, the full-length of VP1 region was amplified by RT-PCR. The EV typing was identified again by VP1 region. Among 1 828 cases of ARIs, 56.7% (1 036) were males. The median ( , ) age was about 3 (1, 5) years. Patients under 5 years old accounted for 71.6% (1 309 cases). Among all case