The influence of knocking down the expression of low-density lipoprotein receptor associated proteins on the vascular abnormalities in hepatocellular carcinoma and its mechanisms

• Published in: Zhōnghuá zhŏngliú zázhì Vol. 46; no. 5; p. 399
• Main Authors: Wu, Q, Zhan, L L, Wang, Y, He, Y C, Chen, L, Chen, Z Z, Li, G T, Liu, D M, Bao, X, Liu, X M, Guo, H, Song, T Q
• Format: Journal Article
• Language: Chinese
• Published: China 23.05.2024
• Subjects: Carcinoembryonic Antigen - genetics; Carcinoembryonic Antigen - metabolism; Carcinoma, Hepatocellular - blood supply; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Gene Knockdown Techniques; Human Umbilical Vein Endothelial Cells - metabolism; Humans; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Neovascularization, Pathologic - metabolism; Platelet Endothelial Cell Adhesion Molecule-1 - genetics; Platelet Endothelial Cell Adhesion Molecule-1 - metabolism; Receptors, LDL - genetics; Receptors, LDL - metabolism; Signal Transduction; Transcriptome
• ISSN: 0253-3766
• DOI: 10.3760/cma.j.cn112152-20230809-00071

To investigate the effect of the expression of low-density lipoprotein receptor associated protein (LDLR) on the vascular abnormalities in hepatocellular carcinoma (HCC) and its mechanisms. Based on the information of Oncomine Cancer GeneChip database, we analyzed the correlation between the expression level of LDLR and the expression level of carcinoembryonic antigen (CEA) and CD31 in hepatocellular carcinoma tissues. Lentiviral transfection of short hairpin RNA target genes was used to construct LDLR-knockdown MHCC-97H and HLE hepatocellular carcinoma cells. The differential genes and their expression level changes in LDLR-knockdown hepatocellular carcinoma cells were detected by transcriptome sequencing, real-time fluorescence quantitative polymerase chain reaction, and protein immunoblotting. The gene-related signaling pathways that involve LDLR were clarified by enrichment analysis. The effect of LDLR on CEA was assessed by the detection of CEA content in conditioned medium of hepatocellular carcinoma ce