Inhibitory effects of 1,3-diaminopropane on the biofilm formation of Pseudomonas aeruginosa via interaction with quorum sensing system

To study the inhibitory effects of 1,3-diaminopropane on the biofilm formation of and the underlying mechanisms. The experiment was divided into an experimental group and a control group. Crystal violet staining was used to examine the inhibitory effects of 1,3-diaminopropane on the biofilm formatio...

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Published inZhong nan da xue xue bao. Journal of Central South University. Yi xue ban Vol. 46; no. 9; pp. 942 - 948
Main Authors She, Pengfei, Zhou, Linying, Li, Shijia, Zeng, Xianghai, Wu, Yong
Format Journal Article
LanguageChinese
English
Published China 28.09.2021
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Summary:To study the inhibitory effects of 1,3-diaminopropane on the biofilm formation of and the underlying mechanisms. The experiment was divided into an experimental group and a control group. Crystal violet staining was used to examine the inhibitory effects of 1,3-diaminopropane on the biofilm formation of , and the biofilm formation was compared between the 2 groups.Initial adherence inhibition assay and swimming plate assay were used to determine the inhibitory effects of 1,3-diaminopropane on the initial adherence and swimming motility of ,and the quantification of adhered cells and swimming diameter were compared between the 2 groups. Meanwhile, Western blotting was used to detect the Flagellin production of ; real-time RT-PCR was used to detect the quorum sensing system relative genes and flagellum regulative related genes expression in the 2 groups. Finally, molecular docking assay was used to calculate the interaction between 1,3-diaminopropane and LasI. Compared with the control group, the biofilm formation of was significantly inhibited in the experimental group in a dose-dependent manner ( =6.07, <0.01).Compared with the control group, the initial adherence of could significantly inhibit from (0.890±0.389)×10 to (0.245±0.076)×10 CFU/mL ( =3.257, <0.05) in the experimental group (2.0 mmol/L).Compared with the control group, the swimming motility of flagellar mediation could also inhibit in the experimental group (2.0 mmol/L). The swimming motility diameter was from (1.840±0.144) to (0.756±0.222) cm ( =7.099, <0.01). Compared with the control group, the Flagellin production was inhibited in the experimental group. Finally, the molecular docking assay showed that the potential target of 1,3-diaminopropane was LasI. 1,3-diaminopropane can significantly inhibit the biofilm formation of , which potentially targets LasI protein.
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ISSN:1672-7347
DOI:10.11817/j.issn.1672-7347.2021.200810