Inhibitory effects of 1,3-diaminopropane on the biofilm formation of Pseudomonas aeruginosa via interaction with quorum sensing system
To study the inhibitory effects of 1,3-diaminopropane on the biofilm formation of and the underlying mechanisms. The experiment was divided into an experimental group and a control group. Crystal violet staining was used to examine the inhibitory effects of 1,3-diaminopropane on the biofilm formatio...
Saved in:
Published in | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban Vol. 46; no. 9; pp. 942 - 948 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | Chinese English |
Published |
China
28.09.2021
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | To study the inhibitory effects of 1,3-diaminopropane on the biofilm formation of
and the underlying mechanisms.
The experiment was divided into an experimental group and a control group. Crystal violet staining was used to examine the inhibitory effects of 1,3-diaminopropane on the biofilm formation of
, and the biofilm formation was compared between the 2 groups.Initial adherence inhibition assay and swimming plate assay were used to determine the inhibitory effects of 1,3-diaminopropane on the initial adherence and swimming motility of
,and the quantification of adhered cells and swimming diameter were compared between the 2 groups. Meanwhile, Western blotting was used to detect the Flagellin production of
; real-time RT-PCR was used to detect the quorum sensing system relative genes and flagellum regulative related genes expression in the 2 groups. Finally, molecular docking assay was used to calculate the interaction between 1,3-diaminopropane and LasI.
Compared with the control group, the biofilm formation of
was significantly inhibited in the experimental group in a dose-dependent manner (
=6.07,
<0.01).Compared with the control group, the initial adherence of
could significantly inhibit from (0.890±0.389)×10
to (0.245±0.076)×10
CFU/mL (
=3.257,
<0.05) in the experimental group (2.0 mmol/L).Compared with the control group, the swimming motility of
flagellar mediation could also inhibit in the experimental group (2.0 mmol/L). The swimming motility diameter was from (1.840±0.144) to (0.756±0.222) cm (
=7.099,
<0.01). Compared with the control group, the Flagellin production was inhibited in the experimental group. Finally, the molecular docking assay showed that the potential target of 1,3-diaminopropane was LasI.
1,3-diaminopropane can significantly inhibit the biofilm formation of
, which potentially targets LasI protein. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1672-7347 |
DOI: | 10.11817/j.issn.1672-7347.2021.200810 |