Huotan Jiedu Tongluo decoction alleviates early atherosclerosis of rabbits by inhibiting eNOS uncoupling pathway

• Published in: Zhongguo zhongyao zazhi Vol. 43; no. 21; p. 4305
• Main Authors: Li, Tong, Zhao, Ming-Jing, Han, Xiao-Wan, Deng, Yue, Zhao, Yi-Zhou, Yang, Tao, Wang, Lei, Wang, Bao-Fu, Lu, Zi-Wen, Cheng, Wen-Kun, Wu, Ai-Ming, Lou, Li-Xia, Zhao, Jiu-Li
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2018
• Subjects: atherosclerosis; tetrahydrobiopterin; endothelial nitric oxide uncoupling; Huotan Jiedu Tongluo decoction; reactive oxygen species
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20180820.004

The aim of this paper was to study the curative effect of Huotan Jiedu Tongluo (HTJDTL) decoction on a rabbit model with early atherosclerosis (AS),and furtherly to explore whether it could inhibit the BH4/eNOS uncoupling ROS or not. Twenty-four Japanese white rabbits were randomly divided into sham operation group, model group, HTJDTL decoction group and atorvastatin group. Rabbit models with early atherosclerosis were established by high fat diet, nitrogen drying and carotid artery balloon injury. The rabbits were sacrificed at 7th days after balloon injury and several parameters were measured. The pathological morphology of the common carotid artery was observed by HE staining. The blood lipids were detected by peroxidase method. The ratio of vascular eNOS dimer and monomer was measured by Western blot. The ELISA and biochemical technology were respectively used for testing BH4 and ROS levels in serum. The results showed that compared with the sham operation group, the model group had mild stenosis of the