Measurement of genital mycoplasma concentration by using a microbiological and molecular-biological methods

• Published in: Žurnal mikrobiologii, ėpidemiologii i immunobiologii no. 2; p. 25
• Main Authors: Ivanova, T A, Gushchin, A E, Gamova, N A, Barkhatova, O I, Rakovskaia, I V
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.03.2014
• Subjects: Colony Count, Microbial - standards; Colony Count, Microbial - statistics & numerical data; Culture Media; Humans; Mycoplasma hominis - genetics; Mycoplasma hominis - growth & development; Mycoplasma hominis - isolation & purification; Mycoplasma Infections - diagnosis; Mycoplasma Infections - microbiology; Polymerase Chain Reaction - standards; Polymerase Chain Reaction - statistics & numerical data; Regression Analysis; Ureaplasma - genetics; Ureaplasma - growth & development; Ureaplasma - isolation & purification; Ureaplasma Infections - diagnosis; Ureaplasma Infections - microbiology; Ureaplasma urealyticum - genetics; Ureaplasma urealyticum - growth & development; Ureaplasma urealyticum - isolation & purification; Urogenital System - microbiology
• ISSN: 0372-9311

Establishment of ratios that would allow to execute recalculation of mycoplasma concentration from CFU/ml and/or CCU/ml into units obtained during PCR analysis--geq/ml. Pure cultures of Mycoplasma hominis, Ureaplasma parvum and Ureaplasma urealyticum were studied by cultural and molecular-biological methods with quantitative evaluation. Studies of initial cultures as well as series of 10-fold dilutions were carried out. 32 experiments in total were carried out. Ratio between geq/ml and CFU/ml for M. hominis was 3.5; geq/ ml and CCU/ml ratio--4.4. Ratio between geq/ml and CCU/ml for U. parvum was 7.1; for U. urealyticum--11.2. Ratios between indexes obtained during quantitative study of pure genital micoplasma cultures by using 2 methods were established.