Association between A-kinase anchor proteins 12 methylation and recurrence of hepatocellular carcinoma

• Published in: Zhōnghuá yùfáng-yīxué zázhì Vol. 51; no. 9; p. 848
• Main Authors: Liu, Z J, Liu, W, Cui, C H, Gu, L K, Xing, B C, Deng, D J
• Format: Journal Article
• Language: Chinese
• Published: China 06.09.2017
• Subjects: A Kinase Anchor Proteins - genetics; Adult; Aged; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - pathology; Carcinoma, Hepatocellular - surgery; Cell Cycle Proteins - genetics; DNA Methylation; Female; Humans; Liver Neoplasms - genetics; Liver Neoplasms - pathology; Liver Neoplasms - surgery; Male; Middle Aged; Promoter Regions, Genetic; Recurrence; AKAP12; DNA methylation; Recurrence; Liver neoplasms
• ISSN: 0253-9624
• DOI: 10.3760/cma.j.issn.0253-9624.2017.09.014

To study the association between the AKAP12 promoter methylation and recurrence of hepatocellular carcinoma. A total of 142 primary liver cancer patients underwent surgery in department of Hepatobiliary surgery in Peking University Cancer Hospital from 2003 to 2009 were selected as subjects in the survey; with the inclusion criteria as hepatocellular carcinoma, no cancer cells were observed in the surgical margin(SM) samples. All patients had neither lymph nor distant metastasis at the time of surgery, and receiving complete follow-up data for at least 3 years. By the end of May 2014, a total of 75 patients had relapsed of whom 71 died and there were no lost. All samples were acquired from the frozen surgical tissues. Genomic DNA was extracted using phenol/chloroform method and performed bisulfite modification following with polymerase chain reaction (PCR). AKAP12 methylation in hepatoma and the corresponding SM samples from 142 patients was determined by denature high-performance liquid chromatography (DHPLC