Angiostrongylus (Parastrongylus) cantonensis on intermediate and definitive hosts in Ecuador, 2014-2017

• Published in: Biomédica Vol. 39; no. 2; pp. 370 - 384
• Main Authors: Solórzano-Alava, Luis, Sánchez-Amador, Francisco, Valverde, Talia
• Format: Journal Article
• Language: English; Spanish
• Published: Colombia 15.06.2019
• Subjects: Angiostrongylus cantonensis - growth & development; Angiostrongylus cantonensis - isolation & purification; Animals; Animals, Wild - parasitology; Disease Reservoirs - parasitology; Disease Vectors; Ecuador - epidemiology; Female; Geography, Medical; Introduced Species; Larva; Life Cycle Stages; Rats - parasitology; Rodent Diseases - epidemiology; Rodent Diseases - parasitology; Snails - parasitology; Species Specificity; Strongylida Infections - epidemiology; Strongylida Infections - transmission; Strongylida Infections - veterinary; Zoonoses; Ecuador; Ecuador; host-parasite interactions; rats; Angiostrongylus cantonensis
• ISSN: 0120-4157
• DOI: 10.7705/biomedica.v39i3.4387

Introduction: Angiostrongylus cantonensis is a serious public health problem and is widely distributed in the country. When the parasite infects the snails, it becomes deeply embedded in their interior and accidentally, it can infect people and other mammals. Objective: To establish the geographical distribution of A. cantonensis intermediate hosts (Achatina fulica) and definitive hosts (Rattus spp.) in Ecuador from 2014 to 2017. Materials and methods: We collected 2,908 A. fulica specimens in 16 provinces using the capture method per unit of effort for 30 minutes. We captured 211 hosts of which 20 were Rattus rattus and 191 R. norvegicus. The specimens were transported to the Instituto Nacional de Salud Pública e Investigación in Guayaquil where the larvae L3 were identified and counted in the tissues of the snail, which were then inoculated in laboratory rats to reproduce the life cycle. In the rodents, the brains, hearts, lung arteries and lungs were dissected, and the parasites were identified by taxonomic morphology. Results: Of the snails harvested, 441 were positive for A. cantonensis (15.2%) and a total of 6,166 L3 larvae were found; 77 (36.5%) specimens of Rattus spp., were infected with A. cantonensis and a total of 220 parasites (L4-L5 larvae and adult worms) were collected. Conclusions: We confirmed the presence of A. cantonensis in several provinces, which ratifies the endemic nature of this zoonosis in the national territory. Rattus spp. specimens constitute the definitive hosts of the parasite, which together with the presence of intermediate hosts, indicates the zoonotic potential of this parasitic infection.