Protective effect of Liujing Toutong Tablets on rats with permanent cerebral ischemia via NF-κB signaling pathway

• Published in: Zhongguo zhongyao zazhi Vol. 48; no. 21; p. 5871
• Main Authors: Yu, Zi-Han, Pei, Ke, Zhao, Ting-Ting, Li, Hong-Chang, Li, Qin-Qing, Zhou, Wen-Jing, He, Wen-Bin, Zhang, Jun-Long
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2023
• Subjects: NF-κB; inflammatory reaction; Liujing Toutong Tablets; ischemic stroke; nerve injury
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20230710.705

This study investigated the neuroprotective effects and underlying mechanism of Liujing Toutong Tablets(LJTT) on a rat model of permanent middle cerebral artery occlusion(pMCAO). The pMCAO model was established using the suture method. Eighty-four male SPF-grade SD rats were randomly divided into a sham operation group, a model group, a nimodipine group(0.020 g·kg~(-1)), and high-, medium-, and low-dose LJTT groups(2.8, 1.4, and 0.7 g·kg~(-1)). The Longa score, adhesive removal test and laser speckle contrast imaging technique were used to evaluate the degree of neurological functional impairment and changes in local cerebral blood flow. The survival and mortality of rats in each group were recorded daily. After seven days of continuous administration following the model induction, the rats in each group were euthanized, and brain tissue and blood samples were collected for corresponding parameter measurements. Nissl staining was used to examine pathological changes in brain tissue neurons. The levels of tumo