Transepithelial transport in vivo and in vitro and anti-inflammatory activity of cannabidiol

This study used Caco-2 cells and normal rats to investigate the in vitro absorption characteristics and in vivo pharmacokinetic characteristics of cannabidiol(CBD) and explore the anti-inflammatory mechanism of CBD. The safe concentration range of CBD was determined by the CCK-8 assay, and then the...

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Published inZhongguo zhongyao zazhi Vol. 49; no. 17; p. 4777
Main Authors Li, Rui, Hao, Rui, Chen, Jue, Lu, Li-Yan, Li, Min, Ruan, Wen-Hui
Format Journal Article
LanguageChinese
Published China 01.09.2024
Subjects
Animals
Anti-Inflammatory Agents - pharmacology
Biological Transport - drug effects
Caco-2 Cells
Cannabidiol - pharmacology
Humans
Male
Rats
Rats, Sprague-Dawley
cannabidiol
anti-inflammatory
pharmacokinetics
Caco-2 cells
transport
uptake
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Summary:This study used Caco-2 cells and normal rats to investigate the in vitro absorption characteristics and in vivo pharmacokinetic characteristics of cannabidiol(CBD) and explore the anti-inflammatory mechanism of CBD. The safe concentration range of CBD was determined by the CCK-8 assay, and then the effects of time, concentration, temperature, endocytosis inhibitors, and transport inhibitors on the transepithelial absorption and transport of CBD were assessed. The blood drug concentration was measured at different time points after oral administration in rats for pharmacokinetic profiling, and the pharmacokinetic parameters were calculated. The Caco-2 cell model of inflammation injury was established with lipopolysaccharide(LPS). The effects of CBD on lactate dehydrogenase(LDH) activity, transendothelial electrical resistance(TEER), and levels of inflammatory cytokines of the modeled cells were exami-ned, on the basis of which the anti-inflammatory mechanism of CBD was deciphered. The results showed that withi
ISSN:1001-5302
DOI:10.19540/j.cnki.cjcmm.20240704.202