Mechanism of tetramethylpyrazine combined with neural stem cells transplantation on cerebral ischemia-reperfusion rats

• Published in: Zhongguo zhongyao zazhi Vol. 49; no. 9; p. 2316
• Main Authors: Qi, Ming-Zhu, Wang, Yan-Qiu, Wang, Dong, Li, Zhuo-Hang, Huang, He-Lan, Lin, Na, Su, Xiao-Hui, Kong, Xiang-Ying
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2024
• Subjects: Animals; Brain Ischemia - drug therapy; Brain Ischemia - metabolism; Brain Ischemia - therapy; Cell Movement - drug effects; Cell Proliferation - drug effects; Chemokine CXCL12 - genetics; Chemokine CXCL12 - metabolism; Doublecortin Protein; Humans; Infarction, Middle Cerebral Artery - therapy; Kelch-Like ECH-Associated Protein 1 - genetics; Kelch-Like ECH-Associated Protein 1 - metabolism; Male; NAD(P)H Dehydrogenase (Quinone) - genetics; NAD(P)H Dehydrogenase (Quinone) - metabolism; Neural Stem Cells - drug effects; Neural Stem Cells - metabolism; Neural Stem Cells - transplantation; NF-E2-Related Factor 2 - genetics; NF-E2-Related Factor 2 - metabolism; Pyrazines - pharmacology; Rats; Rats, Sprague-Dawley; Receptors, CXCR4 - genetics; Receptors, CXCR4 - metabolism; Reperfusion Injury - metabolism; Reperfusion Injury - therapy; Stem Cell Transplantation - methods; migration; tetramethylpyrazine; neural stem cell transplantation; oxidative stress
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20230821.401

This study aimed to investigate the intervention effect of tetramethylpyrazine(TMP) combined with transplantation of neural stem cells(NSCs) on middle cerebral artery occlusion(MCAO) rat model and to explore the mechanism of TMP combined with NSCs transplantation on ischemic stroke based on the regulation of stem cell biological behavior. MCAO rats were randomly divided into a model group, a TMP group, an NSCs transplantation group, and a TMP combined with NSCs transplantation group according to neurological function scores. A sham group was set up at the same time. The neurological function score was used to evaluate the improvement of neurological function in MCAO rats after TMP combined with NSCs transplantation. The proliferation, migration, and differentiation of NSCs were evaluated by BrdU, BrdU/DCX, BrdU/NeuN, and BrdU/GFAP immunofluorescence labeling. The protein expression of stromal cell-derived factor 1(SDF-1), C-X-C motif chemokine receptor 4(CXCR4), as well as oxidative stress pathway proteins nu