Molecular mechanism of Spatholobi Caulis in treatment of ovarian cancer based on network pharmacology and experimental verification

• Published in: Zhongguo zhongyao zazhi Vol. 47; no. 3; p. 786
• Main Authors: Zhu, Shi-Chun, Cai, Jun, Wu, Cheng-Yu, Cheng, Chun-Song
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2022
• Subjects: Drugs, Chinese Herbal - pharmacology; Glycogen Synthase Kinase 3 beta - genetics; Humans; Medicine, Chinese Traditional; Molecular Docking Simulation; Network Pharmacology; Ovarian Neoplasms - drug therapy; Ovarian Neoplasms - genetics; prunetin; molecular docking; network pharmacology; ovarian cancer; Spatholobi Caulis
• ISSN: 1001-5302
• DOI: 10.19540/j.cnki.cjcmm.20211103.703

The present study explored the main active ingredients and the underlying mechanism of Spatholobi Caulisin the treatment of ovarian cancer(OC) by network pharmacology, molecular docking, and in vitro cell experiments. The active ingredients and their predicted targets(AITs) were first acquired online with the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). Theoretical disease targets(DTs) were obtained through professional databases including GeneCards, OMIM, PharmGkb, TTD, and DrugBank. The common targets in the intersection of AITs and DTs were used for the construction of a "drug-ingredient-disease-target" network by Cytoscape 3.7.1. STRING database was used to construct a protein-protein interaction(PPI) network. R 4.0.5 was used for GO and KEGG functional enrichment analyses. Schr9 dinger Maestro was used to perform and optimize the molecular docking and virtual screening.Twenty-three active ingredients of Spatholobi Caulis were screened out, involving 75 OC targe